HPLC (high performance liquid chromatograph ) Instrumentation  

Main components in an HPLC system include the solvent reservoir, or multiple reservoirs, a high-pressure pump, a column, injector system and the detector.

The reservoir holds the solvent, which is referred to as the mobile phase because it moves. There are usually a minimum of two reservoirs in a system, with each holding up to 1000 cc of solvent and usually fitted with a gas diffuser through which helium can be bubbled. A pump is used to generate a specified flow of the mobile phase. Although manual injection of samples is still possible, most HPLCs are now fully automated and controlled by computer. The injector, or auto sampler, introduces the solvent into a phase stream that carries the sample into the high pressure (up to 400 bar) column, which contains specific packing material nee

ادامه مطلب  

HPLC Column | Agilent ZORBAX Eclipse XDB-CN Small Molecule Separations LC Column  

For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings.
Features:
Made from highly pure Rx-SIL
Excellent choice for normal-phase applications with basic compounds
Equilibrates more rapidly than ZORBAX Rx-Sil and is used for many of the same normal-phase applications
To compare technical information and specifications for different phases, see our phase specifications chart.

ادامه مطلب  

principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

ادامه مطلب  

Notes on Cleaning bound Protein from RP HPLC columns  

First, a few comments:

·         Before proceeding with any column regeneration or cleaning procedures, always refer to the specific advice provided by the column manufacturer. Approved maintenance and cleaning instructions can often be found in the product guide which comes with the new column. Their guidelines supersede these!

·         Columns are consumable items. After a suitable amount of use, the time and materials required to regenerate them may cost more than the purchase of a replacement column. Always have a new, spare column on hand.

·         Protect your detector. Before washing or cleaning the column, disconnect the column outlet line and direct the column to waste only.

For RP supports, if buffers have been used, always start by washi

ادامه مطلب  

How to Prevent Mobile Phase Problems in HPLC  

Low sensitivity and rising baselines, noise, or spikes on the chromatogram can often be attributed to the mobile phase. Contaminants in the mobile phase are especially troublesome in gradient elution. The baseline may rise, and spurious peaks can appear as the level of the contaminated component increases. Water is the most common source of contamination in reversed phase analyses. You should use only high purity distilled or deionized water when formulating mobile phases. However, several common deionizers introduce organic contaminants into the water. To remove these contaminants, pass the deionized water through activated charcoal or a preparative C18 column. Use only HPLC grade solvents, salts, ion pair reagents, and base and acid modifiers. Cleaning lower quality solvents is time cons

ادامه مطلب  

Shimadzu CDD-10AVP Conductivity LC Detector  

Handles a Wide Variety of Analysis Options
The CDD-10AVP conductivity detector achieves an even higher level of sensitivity and makes it possible to perform a wide variety of analysis scenarios with a single unit. An option card enables the simultaneous 2-channel measurement of anions and cations, and a suppressor option allows expansion to a suppressor system for ultra-high sensitivity work. Organic acids can be analyzed using Shimadzu's unique post-column pH-buffered electroconductivity method.
Low noise, low drift and wide dynamic range assure proven performance of the Shimadzu VP series HPLC. Data acquired by the CDD-10AVP is transferred to the SCL system controller via an optical fiber interface and are digitally processed by workstation. By adding the optional cell (dual kit NS

ادامه مطلب  

Waters ACQUITY Arc HPLC and UHPLC System  

HPLC and UHPLC method compatibility at the flip of a switch

The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support validated assays.

True plug-and-play method compatibility and the ability to switch between HPLC and UHPLC separations at the flip of a switch with Arc Multi-flow path Technology
Get mass detection, without changing the way you work, with the ACQUITY QDa Detector
Deploy the benefits of 2.x μm particle columns to boost the chromatographic performance of your methods
On-demand mobile phase creation with Auto●Blend Plus Technology, eliminating the cumbersome and error-prone appr

ادامه مطلب  

Partition Chromatography  

Partition chromatography was one of the first kinds of chromatography that chemists developed. The partition coefficient principle has been applied in paper chromatography,thin layer chromatography, gas phase and liquid–liquid separation applications. The 1952Nobel Prize in chemistry was earned by Archer John Porter Martin and Richard Laurence Millington Synge for their development of the technique, which was used for their separation of amino acids. Partition chromatography uses a retained solvent, on the surface or within the grains or fibers of an "inert" solid supporting matrix as with paper chromatography; or takes advantage of some coulombic and/or hydrogen donor interaction with the stationary phase. Analyte molecules partition between a liquid stationary phase and the eluent.

ادامه مطلب  

Waters Breeze 2 HPLC  

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs.
From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization seeking a quality HPLC platform with limited budget and chromatography experience including university laboratories, government laboratories, or start-up companies.
The Waters® Breeze 2 HPLC System delivers routine analyses and robust performance day after day, providing the  onfidence you need to get the job done. Used in laboratories worldwide, chromatograp

ادامه مطلب  

Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes  

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
When some, but not all of your peaks are low, and you are analyzing multiple analytes, the number one rule is to look for trends. As mentioned in the previous post for this series, when you have only one analyte, or a handful of very similar analytes, the following may also apply. Again in this post, the assumption is that you are using an established working method and that you see a loss in response for every sample or standard that is injected. If you are only seeing the problem with samples and not your quantitation

ادامه مطلب  

Features Of Agilent 1290 Infinity II LC System  

Maximize Analytical Efficiency
More chromatographic resolution – specially designed components in the sample flow path achieve lowest system dispersion.
Higher peak capacity for challenging separations ­– switch easily between single dimension UHPLC and the ultimate chromatographic power of 2D-LC.
Lower carryover for uncompromised data quality – multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine.
Unique detection capabilities - combine lowest detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR- DAD or 1290 Infinity II ELSD.
Maximize Instrument Efficiency
Faster injection cycles with dual-needle injection and smart overlapped injections off

ادامه مطلب  

Used Agilent 1260 HPLC with 1260 with Binary pump,DAD,Autosampler, Degasser with solvent tray  

This Agilent 1260 HPLC included:
Binary pump with switch valve
 1260 Binary Pump
 Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD)
 Autosampler (HiP-ALS SL)
 1290 Thermostat for Autosampler
1260 HiP Degasser with solvent tray
 

ادامه مطلب  

Brief History and Definition :What Is HPLC (High Performance Liquid Chromatography)?  

Liquid chromatography was defined in the early 1900s by the work of the Russian botanist, Mikhail S. Tswett. His
pioneering studies focused on separating compounds [leaf pigments], extracted from plants using a solvent, in a
column packed with particles.
Tswett filled an open glass column with particles. Two specific materials that he found useful were powdered chalk
[calcium carbonate] and alumina. He poured his sample [solvent extract of homogenized plant leaves] into the
column and allowed it to pass into the particle bed. This was followed by pure solvent. As the sample passed down
through the column by gravity, different colored bands could be seen separating because some components were
moving faster than others. He related these separated, different-colored bands to the diffe

ادامه مطلب  

AGILENT HP 1200 HPLC Series SYSTEM  

1200 Series HPLC System in excellent working condition.
Includes:
    •    Sold Guaranteed in Good Working Condition
    •    Tested and Verified in Good Working Condition prior to ship
•    G1322A Agilent 1200 Micro Vacuum Degasser
•    Your choice Binary or Quaternary Pump
•    Many AutoSamplers to choose from
•    G1316B Agilent 1200 Thermostatted Column Compartment
•    G1315D Agilent 1200/1260 DAD Diode Array Detector
•    Agilent Solvent tray and bottles
•    All fittings, cables, and tubing
•    Agilent Chemstation Software with License
•    Computer
•    21.5" LCD Monitor
•    Keyboard and mouse
    •    WARRANTY: 3 Months Parts and Labor
INSTALLATION on-site in USA.
Includes:
• Pump sea

ادامه مطلب  

Waters ACQUITY UPLC H-Class System  

With dispersion of less than 10 μL, the ACQUITY UPLC H-Class System delivers the highest resolution of any quaternary LC system in the market. Achieving true UPLC performance requires that system dispersion (or extra-column band spread) be matched to the peak widths associated with narrowbore columns packed with sub-2-μm particles.  While other systems might be designed for high pressure, it doesn’t mean they're designed to deliver the sharp peaks associated with UPLC performance and the ACQUITY UPLC H-Class System.
Highest resolution of any quaternary LC system

Improve your laboratory’s efficiency by scaling your methods to UPLC Technology
Use UPLC’s unmatched resolution to get better characterization of your complex samples
Take advantage of tools that simplify and str

ادامه مطلب  

USED AGILENT HP 1200 HPLC Series SYSTEM  

We offer an Agilent Technologies 1200 Series HPLC System in excellent working condition. Includes:
    •    Tested and Verified in Good Working Condition prior to ship
    •    G1322A Agilent 1200 Micro Vacuum Degasser
    •    Your choice Binary or Quaternary Pump
    •    Many AutoSamplers to choose from
    •    G1316B Agilent 1200 Thermostatted Column Compartment
    •    G1315D Agilent 1200/1260 DAD Diode Array Detector
    •    Agilent Solvent tray and bottles
    •    All fittings, cables, an

ادامه مطلب  

Download Hplc Agilent Technologies 1290 Infinity II LC System Brochure and Guide  

 

Download Agilent InfinityLab LC Series Portfolio Brochure
The Agilent InfinityLab LC Series offers a comprehensive portfolio of LC solutions that give you uncompromised chromatographic performance

Brochure
10-Jun-2016
2.49 MB
PDF


Download Agilent InfinityLab LC Series Selection Guide
Agilent InfinityLab LC Series Selection Guide

Brochure
10-Jun-2016
791.26 KB
PDF


Download Agilent 1290 Infinity II LC – More Efficiency, More Free Time
The Agilent 1290 Infinity II LC is the next generation UHPLC, maximizing analytical, instrument and laboratory efficiency.

Brochure
01-Nov-2016
2.67 MB
PDF

ApplicationsArticle ReprintsBrochures

Download Agilent 1290 Infinity II LC – More Efficiency, More Free Time
The Agilent 1290 Infinity II LC is the nex

ادامه مطلب  

Agilent 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

ادامه مطلب  

Hplc Agilent Technologies 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

ادامه مطلب  

HPLC Refractive-Index (RI) Detector  

RI detector measures change in reflex index. A glass cell is divided into two chambers (cells). The effluent from LC column flow through the "sample cell", while other cell called "reference cell" is filled with only mobile phase. When the effluent going through the sample cell does not contain any analyte, the solvent inside both cells are the same (Figure 1A). When a beam is irradiate on the cells, the observed beam will be straight in this case. However, in a case the effluent contains any components other than mobile phase; bending of the incident beam occurs due to the reflex index difference between the two solvents (Figure 1B). By measuring this change, the presence of components can be observed.

RI detector has lower sensitivity compared to UV detector, and that's the main reason

ادامه مطلب  

History Of Agilent  

Agilent Technologies was created in 1999 by the spin-off of Hewlett-Packard's (HP's) "Medical Products and Instrument Group",[including instrumentation and chemical analysis, electronic component and medical equipment product lines. The split was predicated on the difficulty of growing HP's revenue stream and on the competitive vigor of smaller, more agile competitors.The company's launch slogan was "Innovating the HP Way", which capitalized on the strong HP corporate culture.The starburstlogo was selected to reflect "a burst of insight" (or "spark of insight")and the name "Agilent" aimed to invoke the notion of agility as a trait of the new firm.The Agilent spin-off was accompanied by an initial public offering which raised US$2,100,000,000, setting a record at the time.
2000–2008
In

ادامه مطلب  

What Is Column chromatography?  

For more details on this topic, see Column chromatography.
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.
In 1978, W. Clark Still introduced a modified version of column chromatography called flash column chromatography (flash).The technique is very similar to the traditional column chromatography, except for that the

ادامه مطلب  

Waters Alliance HPLC  

Waters Alliance HPLC THAT WORKS AS HARD AS YOU DO 
Confidence in your results comes with high performance, day-to-day reliability, and overall system robustness. The Alliance® HPLC System was designed to meet demanding method criteria for repeatability, linearity, and accuracy, resulting in data that you can depend on with eacy analysis. Achieving low carryover with a programmable wash routine, delivering reproducible gradients with automatic solvent compressibility and Auto•Blend™  Technology, and streamlining daily start-up with SystemPREP are just some of the ways that the Alliance HPLC System delivers the performance your methods require, making your laboratory more efficient.
Waters Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance H

ادامه مطلب  

Ion Exchange Chromatography / Ion chromatography  

In ion-exchange chromatography (IC), retention is based on the attraction between solute ions and charged sites bound to the stationary phase. Solute ions of the same charge as the charged sites on the column are excluded from binding, while solute ions of the opposite charge of the charged sites of the column are retained on the column. Solute ions that are retained on the column can be eluted from the column by changing the solvent conditions (e.g. increasing the ion effect of the solvent system by increasing the salt concentration of the solution, increasing the column temperature, changing the pH of the solvent, etc...).
Types of ion exchangers include:

Polystyrene resins – These allow cross linkage which increases the stability of the chain. Higher cross linkage reduces swerving,

ادامه مطلب  

Principles Of Operation In Evaporative light Scattering Detector (ELSD)  

ELSDs analyze solvent after elution from HPLC. As the eluent passes from an HPLC, it is mixed with an inert carrier gas and forced through a nebulizer, which separates the liquid into minute aerosolized droplets. These droplets then pass into a heated drift tube, where the mobile phase solvent is evaporated off. As the mobile phase evaporates, the droplets become smaller and smaller until all that is left is minute particles of dried analyte. These particles are pushed through the drift tube by the carrier gas to the detection region. In this region, a beam of light crosses the column of analyte and the scattering of light is measured by a photo multiplier tube./wikipedia

ادامه مطلب  

Used Agilent 1200 Hplc system with Degasser,Bin pump ,Hip-als ,MWD,TCC  

The binary pump comprises two identical pumps integrated into one housing. It provides gradient generation by high-pressure mixing The binary pump is based on a two-channel, dual-piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by two pump assemblies which can generate pressure up to 400 bar.

Each channel comprises a pump assembly including pump drive, pump head, active inlet valve which has a replaceable cartridge, and outlet valve. Both channels are connected in a low-volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer. A purge valve including a PTFE frit is fitted at th

ادامه مطلب  

High performance liquid chromatography (HPLC)  

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.

Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the s

ادامه مطلب  

Used Agilent 1260 LC System with Quaternary Pump and VWD detector  

This  Agilent 1260 LC System with Quaternary Pump and VWD detector included:
1260 Solvent Tray
1260 Quaternary Pump G1311C
1260 Autosampler G1329B
1260 Thermostatted Column Compartment
1260 VWD G1314F with standard Flow Cell
 

ادامه مطلب  

HPLC (high performance liquid chromatograph ) Instrument Operation  

General components of a high performance liquid chromatograph.
HPLC solvent delivery systems.
How automatic injectors work.
Common HPLC detectors.
Downlod powerpoint file about hplc instrumentation .
source:Philadelphia University

ادامه مطلب  

Agilent 1290 Infinity II Refractive Index LC Detector  

The 1290 Infinity II Refractive Index Detector is equipped with an ultralow dispersion microflow cell, which significantly reduces run times for higher sample throughput and improved resolution. Lower solvent consumption means much lower cost of analysis. A high-performance detector of choice for accurate, reproducible, routine analysis of polymers and other compounds that aren’t detectable by UV.
Features Of Agilent 1290 Infinity II Refractive Index LC Detector:
Shorter run times – for considerably higher sample throughput.
Ultralow dispersion – for improved sample definition and resolution.
Reduced solvent consumption – for significant savings in analysis cost.
Ideal tool for polymer analysis – consistent molecular weights, micro or analytical scale.
Excellent sensitiv

ادامه مطلب  

What Is High Performance Liquid Chromatography (HPLC)?  

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

ادامه مطلب  

Used Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD  

This Agilent 1260 HPLC included:
1260 Binary Pump SL and G4225A
1260 HiP Degasser with solvent tray  or G1311B(built-in degasser )
Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD),
 Agilent 1260
Autosampler (HiP-ALS SL)
 1260 Thermostat for Autosampler
If you are interested in Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD,please contact us.

ادامه مطلب  

Chromatography terms / analyte / Analytical chromatography / bonded phase / chromatogram /...  

The analyte is the substance to be separated during chromatography. It is also normally what is needed from the mixture.
Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in asample.
A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.
 Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response creat

ادامه مطلب  

Mobile Phase (eluent) In Column Chromatography  

The mobile phase or eluent is either a pure solvent or a mixture of different solvents. It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. The eluent has also been chosen so that the different compounds can be separated effectively. The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase.
There is an optimum flow rate for each particular separation. A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. However, the maximum flow rate is limited because a finite time is required for the analyte to equi

ادامه مطلب  

Used Agilent 1100 with Binary Pump  

Manufacturer: Agilent Technologies
Condition: As Is
Includes:

1100 Solvent Tray
1100 Binary Pump
1100 Vacuum Degasser
1100 Autosampler
Cables and Manuals
Used Agilent 1100 LC System with Binary Pump & DAD is currently not available for ordering, and it may not be in stock again.

ادامه مطلب  

Used Agilent 1100 LC System with Binary Pump & DAD  

Manufacturer: Agilent Technologies
Condition: As Is
Includes:

1100 Solvent Tray
1100 Degasser
1100 Binary Pump
1100 Autosampler
1100 Diode Array Detector (DAD)
G2170 Data System
HPIB cables and CAN cables
Manuals
Used Agilent 1100 LC System with Binary Pump & DAD is currently not available for ordering, and it may not be in stock again.

ادامه مطلب  
صفحات ادامه نتايج:  1